1. Reconstitution Guide

This is the first step once you have acquired your peptide vial and bacteriostatic (BAC) water.

What is Reconstitution?

"Reconstitution" is mixing a dried powder (peptide) with a liquid (BAC water) to turn it into a liquid solution. You have to "reconstitute" the powder so it can be safely measured and used.

I. The Reconstitution Process

• Wipe the rubber tops of both vials with an alcohol swab.

• Use a syringe to draw up the amount of water you need.

• Slowly squirt the water into the powder vial, aiming the stream at the glass side so you don't blast the powder.

• Gently swirl the vial in circles until it dissolves. Do not shake it!

II. The Math (Calculating Concentration)

To figure out how much liquid to draw up for your dose, you first need to establish the concentration of your newly mixed vial.

The Formula:

Total Compound Weight (mg) ÷ Total Water Added (mL) = Concentration (mg per mL)

Unit Conversion Reference Table

III. Calculating Your Dose in "Units"

Most standard insulin syringes are U-100, meaning 1 mL of liquid equals 100 units on the barrel of the syringe.

The Formula:

(Desired Dose in mg ÷ Concentration in mg per mL) × 100 = Units on Syringe

Practical Example:

• Vial Size: You have a vial containing 5mg of powder.

• Water Added: You add 1mL (100 units) of bacteriostatic water.

• Concentration: 5mg ÷ 1mL = 5mg per mL.

• Desired Dose: You want to take a 0.5mg (which is 500mcg) dose.

• The Calculation: (0.5mg ÷ 5mg) × 100 = 10 units.

To get your desired 0.5mg dose, you would draw the liquid up to the 10 mark on a standard U-100 insulin syringe.

2. Aseptic Sterilization

How to Inject After Reconstitution

• I. Sanitize the vial lid: Whenever a needle is going to puncture something, you must sanitize it first with an alcohol swab. Lightly rub the surface of the peptide vial's lid (not the needle) with the swab and wait for it to dry.

• II. Draw your dose: Pull the properly calculated liquid dose into your syringe (such as an insulin syringe) based on the concentration of your newly reconstituted vial.

• III. Sanitize your skin: Swab the target injection area on your body and let the alcohol dry.

• IV. Select your injection site: Subcutaneous (SubQ) is highly recommended, largely considered more comfortable, and typically done in abdominal, upper thigh, or upper glute fat. Intramuscular (IM) injections are often unnecessary for water-based peptides, but the rule of thumb is "if you can flex it, you can inject it".

• V. Inject the compound: Administer the peptide into the subcutaneous fat or muscle for proper absorption, as drinking peptides will not do much. Video tutorials for both SubQ and IM styles of injection are available online.

• VI. Dispose of the equipment: Put the cap on your needle and dispose of it responsibly. You must NOT reuse needles.

3. Storage Guidelines

While storage conditions do not matter as much as commonly believed — most peptides resist degradation well — it is still good practice to maximize shelf life.

• Powder vials: Store in the freezer.

• Reconstituted powder vials: Once reconstituted, they become water-based and must be stored in the fridge.

• Water-based injectables: Store in the fridge. Examples include L-carnitine, L-glutathione, and injectable aminos.

• Oil-based compounds: Store at room temperature. Examples include Testosterone, boldenone, and primobolan.

• Everything else: Store at room temperature. Compounds like Cardarine and MK-677 never really degrade.

⚠️ Improper storage will cause products to degrade faster. Because there are no exhaustive studies on every individual peptide's shelf-life, users may need to experiment to determine it themselves — though many peptides do not degrade very fast.